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. Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Google Scholar Summary Tobolski J.

Identification of red maple cultivars by isozyme analysis. Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue ( Festuca pratensis Huds. M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms.

Proc Natl Acad Sci USA 85: 9176–9178. Google Scholar Not logged in Not affiliated 37. DNA variation in tissue-culture-derived rice plants.

Theor Appl Genet 80: 673–679.

In vitro propagation of Alstroemeria ‘Alsaan’. Plant Cell, Tissue and Organ Culture 9: 29–35. Google Scholar We have used random amplified polymorphic DNA (RAPD) markers to study genetic variation in Alstroemeria.

The first objective was to examine the discriminatory power of RAPD markers in different genotypes of Alstroemeria obtained by traditional breeding. All genotypes examined, including commercial Alstroemeria varieties, could be distinguished on the basis of their RAPD profiles. Progeny plants could be distinguished from their parents.

A second objective of this study was to investigate whether RAPD markers can be used as a routine tool to detect mutant plants, as an alternative to glasshouse testing. To address this objective, we analysed Alstroemeria plants that carried phenotypically visible mutations that either were induced by irradiation using X-rays or were the result of somaclonal variation. In eight out of a total of 13 mutant Alstroemeria plants obtained after irradiation or tissue culture we detected no polymorphisms when compared to control plants that were considered to be non-mutated.

Only in five of the mutant plants analysed we detected one to two polymorphisms

. These results suggest that frequent genome rearrangements had not occurred in the mutant plants analysed. These results also demonstrate that the RAPD technique is an inappropriate tool for the rapid screening of Alstroemeria for induced variation.

It that the RAPD technique is an inappropriate tool for the rapid screening of Alstroemeria for induced variation. It seems probable that this conclusion would be equally applicable in other plant genera in which induced variation has occurred. However, the RAPD technique is a simple and effective tool for genetic fingerprinting of Alstroemeria varieties, provided their differences are due to sexual propagation. Determination of genetic stability using isozymes and RFLPs in beet plants regenerated in vitro.

Theor Appl Genet 84: 113–117. Isozyme studies in provenance research of forest trees. Theor Appl Genet 69: 335–347


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  • 19 Citations Larkin P. Somaclonal variation-a novel source of variability from cell cultures for plant improvement.

    Theor Appl Genet 60:, 197–214. Mutation breeding of Alstroemeria. Isozyme characterization in Alstroemeria species and cultivars. RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill. Google Scholar January 1996, Volume 90, Issue 2,

  • 71 Downloads Kuhns L.

    Distinguishing rose cultivars by polyacrylamide gel electrophoresis. Extraction and storage of protein and active enzymes from rose leaves. Google Scholar

  • Accepted 23 January 1996 Sambrook, J.

    Molecular Cloning: A Laboratory Manual. Optimizing the generation of random amplified polymorphic DNAs in chrysanthemum.

    Theor Appl Genet 86: 1033–1037. Forestry Research Unit Shell Research Limited U. Molecular genetic markers and their legal relevance for cultivar discrimination: a case study in cucumber. Excess of non-parental bands in offspring from known primate pedigrees assayed using RAPD PCR. Use of restriction fragment length polymorphism to fingerprint beets at the genotype and species levels.

    Theor Appl Genet 78: 847–851.

    Sensitivity of random amplified polymorphic DNA analysis to detect change in sugar cane during tissue culture. Theor Appl Genet 90: 1169–1173. Detection of DNA “fingerprints” of cultivated rice by hybridization with a human minisatellite probe.

    Proc Natl Acad Sci USA 85: 6831–6835. Genetic markers for Prunus avium L. Clonal identifications and discrimination from P. Molecular characterization of UV-treated sugar beet somaclones using RFLP markers.

    Physiologia Plantarum 90: 216–220. Oligonucleotide fingerprinting in plants and fungi.

    ), DNA Fingerprinting Approaches and Applications, pp. Google Scholar Key words Alstroemeria genetic fingerprinting RAPD somaclonal variation alstroemeria Caetano-Anollés G. DNA amplification fingerprinting using very short arbitrary oligonu-cleotide primers.

    Google Scholar Gonzalez-Benito M. Callus induction and plant regeneration in Alstroemeria. Cluster Analysis for Applications. Google Scholar © Kluwer Academic Publishers 1996 Authors and Affiliations Nordenstam B.

    Pilgrim from Lima-the route of alstroemeria to Sweden. Fauna-Flora (Stockh) 73: 134–136.

    Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Isolation of plant DNA from fresh tissue.

    Identifying rose cultivars using random amplified polymorphic DNA markers. Selective restriction fragment amplification: a general method for DNA fingerprinting.

    European Patent Application, No.

    Somaclonal variation: genetic basis and breeding applications. Clonal fidelity of norway spruce somatic embryos analyzed by RAPD. ), Biotechnology of Trees, Conf.

    Google Scholar Euphytica Loubaka L.

    RAPD markers for use in discrimination and verification of genotypes in Eucalyptus-Training of a Congolese forester in RAPD technology.

    ), Eucalypt Plantations: Improving Fibre Yield and Quality, pp. CRC-IUFRO Conf Hobart, 19–24 Feb


    , CRC fro Temperate Hardwood Forestry: Hobart, Australia.

    Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Polymorphic simple GATA/GACA repeats in plant gnomes


    Genome modifications in protoplast-derived tobacco plants: phenotypic evaluation and RFLP analysis. Biologia Plantarum (Praha) 33: 455–460. Google Scholar Assessment of natural and induced genetic variation in Alstroemeria using random amplified polymorphic DNA (RAPD) markers | SpringerLink pp 235–244 | Cite as Pierik R. Vegetative propagation of Alstroemeria hybrids in vitro. Biochemical and molecular analysis of plants derived from embryogenic tissue cultures of napier grass ( Pennisetum purpureum K. Theor Appl Genet 83: 947–955. , (1989) DNA methylation in plants and its role in tissue culture. Identification of broccoli and cauliflower cultivars with RAPD markers. RFLP analysis of Zea mays callus cultures and their regenerated plants. Theor Appl Genet 81: 227–232.

    DNA polymophisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535. Hortus Third: A Concise Dictionary of Plant Cultivars in the United States and Canada. Google Scholar

  • DOI https://doi.


  • Publisher Name Kluwer Academic Publishers
  • E. Use of random amplified polymorphic DNA (RAPD) markers in the discrimination and verification of genotypes in Eucalyptus.

    Theor Appl Genet 89: 442–450. Google Scholar Anastassopoulos, E.


  • Received 14 July 1995 Nybom H. (1991) Applications of DNA fingerprinting in plant breeding. ), DNA Fingerprinting Approaches and Applications, pp. Birkhäuser Verlag, Basel, Switzerland. Google Scholar Assessment of natural and induced genetic variation in Alstroemeria using random amplified polymorphic DNA (RAPD) markers

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