How do you prepare hibiscus for winter?
Use of calcium chloride in postharvest treatment of Alstroemeria cut flowers. Acta Horticulturae (ISHS), 1104(41):267-272, 2015.
(2001) also observed that the treatment with 1-MCP at 0. 5 g m -3 for six hours, provided an increase in carotenoid content in roses, Consolida ajacis, Antirrhinum majus, gypsophila, alstroemeria, lilies and poinsettias, being the results in agreement with those found in this experiment.
Storage temperature affects the quality of cut flowers from the Asteraceae. Polyfenoloxydase from starfruit. Pertanika Journal, 9(2):219-224, 1986. Manutenção da qualidade de rosas cortadas cv.
Vega em soluções conservantes. Horticultura Brasileira, 30(1):64-70, 2012. The commercial storage of fruits, vegetables and floristand nursery stocks.
Agriculture Research Service, Canada, 1990. The longevity of a plant depends on the respiratory activity, because it is related to the growth and senescence, therefore its monitoring is important once if it is not checked, can compromises the quality, shortening the life of the plant.
Figure 4: Respiratory rate in stems of alstroemerias ‘Ajax’ exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH. Means followed by at least one common letter, on each day of analysis, do not differ by Tukey test (P>0.
Vertical bars represent the standard error of the mean.
Postharvest physiology of perishable plant products. New York: Van Nostrand Reinhold, 1991.
Among the applied treatments the dose of 500 ppb 1-MCP reduces the loss of water of the stems of Alstroemeria cv. Ajax during the storage period, keeping the petals turgids and the levels of carotenoids and anthocyanins high. However, this was not enough to keep the decorative quality of the flowers, once the 1-MCP did not solve the problems of yellowing of the leaves and floral openning, which are important characteristics for its commercialization.
Carlos Orlando Inestroza-Lizardo 3 2Universidade Estadual Paulista “Júlio de Mesquita Filho”/UNESP, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, SP, Brasil
Mitochondrial regulation of cell cycle and proliferation. Antioxid Redox Signal, 16(10):1150-1180, 2012. Claudia Machado Fabrino Mattiuz 4 MATTIUZ, C.
Efeito de agentes químicos na conservação pós-colheita de inflorescências de Alpinia purpurata (Vieill) K. Revista Brasileira de Plantas Ornamentais, 11(4):35-42, 2005.
New York: Academic Press , 1983, 489p. The effect of natural essential carvacrol and some growth regulators on vase life of Alstroemeria cut flowers. Journal of Ornamental Plants, 4(2):115-122, 2014.
Due to the scarce informations related to postharvest physiology of alstroemeria cut flowers, this study aimed to verify the best dose of 1-methylcyclopropene to the postharvest conservation of inflorescences of Alstroemeria cv. Ajax, considering the factors associated with floral senescence and loss of the decorative life. Carbohydrate and ethylene levels regulate citrus fruitlet drop through the abscission zone a during early development. Role of ethylene and 1-MCP in flower development and petal abscission in zonal geraniums. HortScience , 36(7):1305-1309, 2001.
In cut roses it has been reported that some stems are incapable of absorbing sufficient amount of the solution, reducing the water potential of the cells of the petals, which can lead to increased levels of osmotically active substances in all cellular compartments ( Wouter; Van Doorn, 2001). Ana Carolina Corrêa Muniz 2 LICHTENTHALER, H.
Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148(22):350-382, 1987. The accumulation of carbohydrates in the cell may also be a mechanism to reduce cellular water potential, promote the absorption of water and consequently the turgor, requiring high amount of water for the development of flower buds to the stadium of full opening ( Iglesias et al. The Alstroemeria stems were placed inside hermetic chambers and exposed to the 1-MCP in gaseous form, for a period of 6 hours, at the following concentrations: 0 (control); 100; 250 and 500 ppb. Subsequently, they were transferred to Erlenmeyer flasks containing 500 mL of distilled water.
For each treatment were used three replications with three stems each. The flowers were stored at room temperature (22 ± 3 ºC) and 85 ± 4% RH and the experiment was conducted in a completely randomized design. Several factors contribute to the acceleration of senescence of the cut flowers, such as transpiration, high respiratory rates, blocking of the xylem vessels, reduced supply of carbohydrates and production and sensitivity to ethylene ( Celikel; Reid, 2002; 2005). Physiological behavior peroxidase isozynes in sweet potato root tissueinjuried by cuting or black rot.
Plant and Cell Physiology, 13(6):1091-1101, 1972. Longevidade pós-colheita da orquídea Epidendrum ibaguense. Revista Brasileira de Horticultura Ornamental, 13(1):31-37, 2007.
37200-000 Lavras, MG-Brasil Figure 2: Relative water content in ‘Ajax’ alstroemerias stems exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH. Means followed by at least one common letter, on each day of analysis, do not differ by Tukey test (P>0.
Vertical bars represent the standard error of the mean. Figure 6: Activity of the enzymes peroxidase (A) and polyphenoloxidase (B) in stems of Alstroemeria cv. ‘Ajax’ exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH.
Vertical bars represent the standard error of the mean. Vanessa Cury Galati 1 * To generate the 1-MCP in gaseous form, the commercial product Ethylbloc ® was used in the form of wettable powder containing 0.
The decline in respiration rate at the end of the storage period occurred simultaneously with the loss of water and the increase in the symptoms of senescence. This indicates a possible disruption of the structure of the membranes and the cytoplasmic organelles that occur during the process of senescence, resulting in decreased respiratory rate ( Arciuch, 2012). This is supported by Hardenburg, Watada and Wang (1990) that argue that the cut flowers have a very limited life after harvest, surviving from its own reserves, which are usually reduced because of morphological and physiological features of tissues.
In the days of storage it was observed a trend of reduction of the respiration rate, even though the statistics were not compared among days of evaluation. 4Universidade de São Paulo/USP, Escola Superior de Agricultura “Luiz de Queiroz”/ESALQ, Piracicaba, SP, Brasil MATTIUZ, C. Water relations of cut inflorescences of Alpinia purpurata treated with seven pulsing solutions.
Acta Horticulturae , 1(683):363-368, 2005. 1-methylcyclopropene: A review.
Postharvest Biology and Technology, 28(1):1-25, 2003. The reducing carbohydrate content showed a reverse trend, that is, there was a reduction of these sugars during storage. Nevertheless, it is apparent that the stems treated with 100 and 250 ppb of 1-MCP were those which maintained the highest values in the last days of storage ( Figure 5B).
The control treatment was the one that had the lowest concentrations of sugars in the petals of the flowers. Figure 3: Qualitative characteristics of ‘Ajax’ alstroemerias stems exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH. Vertical bars represent the standard error of the mean.
Termos para indexação: Etileno; longevidade; pigmentos; atividade enzimática. The concentration of carotenoids found in the flowers was about ten times larger than the concentration of anthocyanins ( Figure 7A and 7B). The carotenoid content increased during storage period for all treatments, and the treatment of 500 ppb of 1-MCP was the one that provided the greatest average for both variables.
This treatment was also the one that achieved the highest anthocyanin content, with the greatest concentration on the 12 th day of storage. To the floral longevity it was separated a set of stems with their respective treatments to evaluate the following parameters of the decorative quality of Alstroemeria flowers: floral opening, color of leaves, anthers falling and turgidity of flowers. For this evaluation was given a rating scale ranging from 5 to 1, where: Note 5 (maximum score): the flowers were 100% closed and 100% turgid, the leaves were 100% green, and had 100% of the anthers; Note 4: 75% of flowers opened and 75% turgid, leaves 75% green and had 75% of the anthers; Note 3: the flowers were 50% opened and 50% turgid, the leaves 50% green and 50% had anther; Note 2: 25% of flowers opened and 25% turgid, 25% of green leaves and had 25% of the anthers; Note 1: 100% of flowers opened and wilted and the leaves completely yellowed, and there was no presence of anthers on the flowers, according to the methodology described by Galati et al. The completion of this evaluation occurred when the flowers presented darkening, abscission, withered petals and yellowing of the leaves. Pós-colheita de frutos e hortaliças: Fisiologia e manuseio.
1Centro Universitário de Votuporanga/UNIFEV, Votuporanga, SP, Brasil FERRANTE, A. Thidiazuron - A potent inhibitor of leaf senescence in Alstroemeria.
Postharvest Biology and Technology , 25(3):333-338, 2002. Influência do 1-MCP na conservação pós-colheita de rosas cv.
Ciência e Agrotecnologia, 34(5):1176-1183, 2010. Longevity of Oncidium varicosum (Orchidaceae) inflorescences treated with 1- methylciclopropene.
Ciência Rural , 42(6):987-992, 2012. The respiratory activity in the alstroemerias stems progressively decreases during the storage period. Among the treatments, stems treated with 100 ppb of 1-MCP had lower respiration rate when compared to the other concentrations ( Figure 4).
The increase in the respiration rate observed in this research may be due to the chemical stress caused by the application of higher doses of 1-MCP ( Rosa et al. According to Dong, Lurie and Zhou (2002), the reduction in the respiration rate promoted by 1- MCP can result from an effect in the carbohydrates metabolism, proving to be more efficient in the maintenance of the contents during all postharvest period, as it was observed in this experiment.
The content of soluble carbohydrates in the petals of Alstroemeria flowers increased during the period in which the plants remained stored. After 12 days of storage the flowers that had the highest levels were the ones treated with 500 ppb of 1-MCP ( Figure 5A). The increase in the soluble carbohydrate content may be related to the increase in the carotenoids and anthocyanins content in the flowers, because the sugars are essential for the metabolism of the plants, also being associated with senescence of the flowers and increase in the pigments contents ( Pietro et al.
Decline in ascorbate peroxidase activity a prerequisite factor for tepal senescence in gladiolus. Journal of Plant Physiology, 163(2):186-194, 2006. Principles and procedures of statistics: A biometrical approach. Armazenamento refrigerado de inflorescências cortadas de Oncidium varicosum ‘Samurai’, Ciência Rural, 40(11):2288-2293, 2010. 1-MCP blocks ethylene-induced petal abscission of Pelargonium peltatumbut the effect is transient. Postharvest Biology and Technology , 22(2):169-177, 2001.
Figure 5: Soluble (A) and reducing (B) carbohydrate content in stems of Alstroemeria cv. ‘Ajax’ exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH. Vertical bars represent the standard error of the mean.
The results were submitted to analysis of variance (ANOVA) and the effect of the treatments, when significant, to the F Test. The means were compared by Tukey Test (p≤0.
When differences between treatments were greater than the sum of the standard deviation, the result was considered significant ( Steel; Torrie, 1987). The loss of water, or water stress during the days of life in the vessel, begins when the water content in the intracellular tissues moves away from the optimum. The water deficit can result in growth reduction, discoloration of flowers, reduction of the turgidity, increase of the susceptibility to physiological disorder caused by cold and acceleration of senescence symptoms ( Kays, 1991).
1590/1413-70542017412032816 January 23, 2017 Delays in floral senescence using 1-MCP were observed in many species, such as alpinias ( Mattiuz, 2005), roses ( Pietro; Mattiuz; Mattiuz, 2010), oriental lilies ( Celikel et al. , 2002), geraniums ( Cameron; Reid, 2001; Jones et al. , 2001) and orchids ( Moraes et al. Serek, Sisler and Reid (1995) observed that the use of 1-MCP increased the vase life of Alstroemeria cut flowers. According to those authors this is one of the cut flowers that respond to ethylene by the petals abscission.
This is an open-access article distributed under the terms of the Creative Commons Attribution License NGUYEN, T.
Relationship between browning and the activities of polyphenoloxidase and phenylalanine ammonialyase in banana peel during low temperature storage. Postharvest Biology and Technology , 30(2):187-193, 2003. Ethylene and flower longevity in Alstroemeria: Relationship between tepal senescence, abscission and ethylenebiosynthesis. Journal of Experimental Botany, 56(413):1007-1016, 2005.
Figure 7: Carotenoid (A) and anthocyanins (B) contents in stems of Alstroemeria cv. ‘Ajax’ exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH. Vertical bars represent the standard error of the mean.
João Emmanuel Ribeiro Guimarães 2 Every three days were evaluated the variation of fresh mass; the relative water content ( Kramer, 1983); the levels of carotenoids and anthocyanins ( Francis, 1982; Lichtenthäler, 1987); the levels of soluble and reducing carbohydrates ( Mattiuz et al. , 2010); the activity of the enzymes polyphenol oxidase and peroxidase ( Adnan; Augustin; Ghazali, 1986; Matsuno; Uritani, 1972). Index terms: Ethylene; longevity; pigments; enzyme activity.
How to cite this article To carry out this research it were used Alstroemeria cut flowers cv. Ajax of red color, grown in Andradas, Minas Gerais, Brazil (22º4’19 “S and 46º34’20” W), whose stems were collected in the morning at the maturation point where the primary flowers showed the color and were about to open. Then, they were transported under refrigeration to the Laboratory of Postharvest of the FCAV-UNESP, campus of Jaboticabal-SP, where they were standardized at 70 cm in length and the leaves that were in contact with the solutions were removed.
Depending on age and water stress caused in the plants, sugars can be located, mostly in the vacuoles and not in the cytoplasm, thus, the mitochondria may not be able to use the sugars, or the cytosol of take them to the mitochondria, occurring accumulation of soluble sugars in the petals of cut flowers during the storage period ( Wouter; Van Doorn, 2001).
A survey of ethylene contamination in Ontario’s floriculture industry and the evaluation of 1-methylcyclopropene and an ethylene absorber as potential solutions. Acta Horticulturae , 543(5):55-62, 2001. Effects of l-MCP on the vase life and ethylene response of cut flowers. Plant Growth Regulate, 16(4):93-97,1995. Postharvest handling of australian flowers from australian native plants and related species, a practical workbook.
Rural Industries Research and Development Corporation, Australian, n.
RESULTS AND DISCUSSION Figure 1: Variation of fresh mass, in percentage, of ‘Ajax’ alstroemerias stems exposed to different concentrations of 1-MCP and maintained at 22±3 ºC and 85±4% RH during 12 days. Vertical bars represent the standard error of the mean. INTRODUCTION 3Universidad Nacional de Agricultura, Departamento de Producción Vegetal, Catacamas, Honduras The activity of the peroxidase enzyme increased during the storage of the flowers.
The ones treated with 500 ppb were those with the highest activity starting on the 6 th day ( Figure 6A). (2006), the activity of peroxidase enzymes is regulated and previously programmed by the increase in the levels of peroxides in the cells, which seems to be a prerequisite for the initiation of senescence in gladiolus. Qualidade físico-química e sensorial de cultivares de morango durante o armazenamento refrigerado. Boletim de Pesquisa e Desenvolvimento, Embrapa Clima Temperado. As flores de corte se deterioram muito rapidamente e necessitam de tecnologias pós-colheita para aumentar a durabilidade, que é normalmente em torno de 8 dias.
Devido às escassas informações relacionadas à fisiologia pós-colheita de flores de Alstroeméria, a presente pesquisa teve como objetivo verificar qual a melhor dose de 1-metilciclopropeno (1-MCP) na conservação pós-colheita de inflorescências de Alstroemeria cv. Ajax, considerando os fatores associados à senescência floral e perda da vida decorativa. As hastes foram submetidas a quatro doses de 1-MCP (0; 100; 250; 500 ppb), posteriormente foram colocadas em recipientes com água destilada e armazenadas em temperatura de ambiente (22 ºC).
Os tratamentos foram realizados em triplicata contendo três hastes por repetição. As avaliações foram realizadas a cada três dias perfazendo um total de 12 dias de armazenamento. Foi realizado um teste de F, e as médias comparadas pelo teste de Tukey ( P≤0,05).
Entre os tratamentos aplicados a dose de 500 ppb de 1-MCP reduziu a perda de água das hastes de alstroeméria durante o período de armazenamento, mantendo as pétalas túrgidas e os níveis de carotenoides e antocianinas altos, entretanto, isso não foi suficiente para manter a qualidade decorativa das flores, uma vez que o 1-MCP não resolveu os problemas de amarelecimento das folhas e abertura floral, que são importantes características para sua comercilização. Ultrastructure in carotenoids composition of chromoplasts of the sepals of Strelitzia reginae Aiton during floral development.
Annals of Botany, 39(2):175-183, 1975. Influence of ethephon, silver thiosulfate and sucrose pulsing on bird of paradise vase life. Revista Brasileira de Fisiologia Vegetal, 11(2):119-122, 1999.
Ben-Hur Mattiuz 2 According to Cantillano et al. (2008), the loss of water is a senescence accelerator, causing an increase in the rate of disintegration of the membrane and loss of cell content, with consequent loss of turgor. The smallest loss of fresh mass may be due to the 1-MCP capacity of blocking the ethylene action and retard the senescence ( Pietro; Mattiuz; Mattiuz, 2010).
Furthermore, the application of 1-MCP can increase the water absorption of the inflorescences, indicating greater turgidity of the flowers during the storage period ( Obsuwan; Uthairatanakij, 2007). For the floral longevity it was observed that the maximum flower opening was reached on the sixth day after storage for all treatments ( Figure 3). From this date, it was evident that the use of 1-MCP did not delay the yellowing of the leaves, moreover, the fall of the anthers began, indicating that in the case of alstroemerias the 1-MCP did not presented significative effect on the chlorophyll degradation in the leaves, what was also observed in rocket leaves ( Koukounaras; Siomos; Safakiotans, 2006).
The stems of the treatment with 500 ppb, from the ninth day of storage, began to present loss of turgor superior to the other treatments, indicating the onset of senescence of the plant.
Effect of 1-MCP on the postharvest quality of the orange cv. Pera store under refrigeration. Revista Ciência Agronômica, 47(4):624-632, 2016.
Role of soluble carbohydrates in flower senescence: A survey. Acta Horticulturae , 543(6):179-183, 2001. Conservação pós-colheita de alstroémeria ‘ajax’ com uso de 1-metilciclopropeno According to Simpson, Baquar and Lee (1975), the increase of carotenoids and anthocyanins is related to the maturity of the flowers and not with the degeneration of the plant structure, which was observed by these researchers in Strelitzia reginae.
Cut flowers deteriorate quickly and require postharvest technologies to increase their durability, which is usually around 8 days. Due to the scarce information related to postharvest physiology of Alstroemeria cut flowers, this study aimed to verify the best dose of 1-methylcyclopropene (1-MCP) to the postharvest conservation of inflorescences of Alstroemeria cv. Ajax, considering the factors associated with floral senescence and loss of the decorative life.
The stems were submitted to four doses of 1-MCP (0; 100; 250; 500 ppb), then placed in containers with distilled water and stored at room temperature (22 ºC). The treatments were performed in triplicate containing three stems per replicate. The evaluations were performed every three days for a total of 12 days of storage.
An F test was conducted, and the means were compared by the Tukey test (p≤0. Among the applied treatments the dose of 500 ppb of 1-MCP reduces the loss of water of the stems of alstroemerias during the storage period, keeping the petals turgids and the levels of carotenoids and anthocyanins high, however, this was not enough to keep the decorative quality of the flowers, once the 1-MCP did not solve the problems of yellowing of the leaves and floral openning, which are important characteristics for its commercialization. 1-Methylcyclopropene prevents ethylene induced yellowing of rocket leaves. Postharvest Biology and Tecnology, 41:109-111, 2006. Effect of 1-methylcyclopropene on ripening of ‘Canino’ apricots and ‘Royal Zee’ plums. Postharvest Biology and Technology , 24(2):135-145, 2002.
These results are in agreement with those found for fresh mass ( Figure 1), being a determining factor in the maintenance of the quality of the plants. Alstroemeria stems treated with 1-MCP at 100 and 500 ppb absorbed the distilled water during all days of storage. In contrast, the control and the 250 ppb treatment started losing fresh mass from the 3 rd day of storage ( Figure 1).
Ethylene is a plant growth regulator produced by plants that affects the longevity of cut flowers, because it induces many physiological responses such as wilting and abscission of leaves, petals and sepals, thus anticipating the senescence of these flowers ( Finger et al.
(2002), the harmful effects of ethylene are responsible for approximately 30% of the losses in the floriculture sector. (2012) studying the application of different concentrations of 1-MCP in Oncidium ‘Samurai’ found that the highest concentration (1000 ppb) delayed the opening of the buds and kept the turgidity of the flowers throughout the storage period.
This was not observed in this experiment, since the application of the ethylene inhibitor did not affect the floral opening ( Wagstaff et al. It is possible that the use of higher concentrations of 1-MCP would delay the flower opening.